Eukaryotic Transcription Quiz
1. In eukaryotic species, three separate RNA polymerases transcribe different categories of genes. RNA polymerase III is known to primarily transcribe:
TRANSFER RNAS AND SMALL NUCLEAR RNAS
2. The carboxyl tail domain of RNA polymerase II plays a key role in:
CAPPING OF THE 5’ END OF A NEW TRANSCRIPT, RECRUITING CAPPING ENZYMES TO THE RNA POLYMERASE ENZYME, ADDITION OF A POLYA TAIL AT THE 3’ END OF A TRANSCRIPT, AND SPLICING OF INTRONS OUT OF RNA TRANSCRIPTS
3. For protein encoding genes, six general transcription factors (GTFs) function to:
IDENTIFY A GENE’S PROMOTER, FACILITATING RNA POLYMERASE II BINDING
4. What is the function of the TATA binding protein?
ALLOWS EUKARYOTIC RNA POLYMERASE II TO BIND TO THE PROMOTER OF GENES
5. In eukaryotes, transcriptional gene control is mediated by
TRANS-ACTING FACTORS BINDING TO CIS-ACTING ELEMENTS
6. Which of the following statements about histones is true?
THEY ARE PROTEINS WHOSE SEQUENCE IS HIGHLY CONSERVED IN ALL EUKARYOTES AND THEY ARE THE BUILDING BLOCKS OF NUCLEOSOMES
7. The ___ is a cis-acting element that binds RNA Polymerase II.
8. The ___ is a cis-acting element that binds trans-acting DNA-binding proteins.
PROMOTER, ENHANCER, PROMOTER-PROXIMAL ELEMENT, AND GC-RICH BOX
9. Which of the following describes the histones associated with the nucleosomes of active genes?
RICH IN ACETYLY GROUPS (I.E., HYPERACETYLATED)
10. The GAL4 protein has which of the following functional domains?
A DNA-BINDING DOMAIN AND AN ACTIVATION DOMAIN
11. GAL80 inhibits GAL gene expression by
SPECIFICALLY BINDING TO GAL4 AND PREVENTING ACTIVATION
12. What is an enhancesome?
A LARGE COMPLEX THAT ACTS SYNERGISTICALLY TO ACTIVATE TRANSCRIPTION